Abstract for presentation at 11th International Congress of Human Genetics

Vitamin D receptor gene alleles predict the bone density and bone turnover

  • Arash Hossein-nezhad, Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences, Iran
  • Zhila Maghbooli, Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences, Iran
  • Vahid Haghpanah, Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences, Iran
  • Samira Shirzad, Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences, Iran
  • Samira Arami, Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences, Iran
  • Habibollah Ghaffari, Hematology -Oncology and BMT Research Center, Iran
  • Bagher Larijani, Endocrinology and Metabolism Research Center of Tehran University of Medical Sciences

    • Aims: To determine association of vitamin D receptor (VDR) gene polymorphisms with bone mineral density (BMD) and bone turnover in familial osteoporosis.
    Methods: We studied allelic frequencies of the FokI,BsmI, ApaI, and TaqI restriction fragment length polymorphisms (RFLPs) in 38 osteoporotic patients (enrolled from 1035 osteoporotic patients )in comparison with 40 age-matched healthy pre- and postmenopausal women aged between 30 and 65 years. Affected criteria were include: T-score <-2.5 , Z-score <-1 and have positive history of familial osteoporosis (in two generations).
    Genomic DNA was isolated from peripheral blood leukocytes . After an overnight fast, blood was taken for measurement of serum parathyroid hormone, 25-hydroxyvitamin D, alkaline phosphatase, osteocalcin and cross laps. Bone measurements were performed with the same instrument in two regions .
    Questionnaire was filled for all of participants that included information about general characteristics, dietary habits, drug, lifestyle, past medical history, sunlight exposure, menopausal status, age at menarche, age at menopause and other factors affecting bone metabolism. Calcium and vitamin D intake estimated from a detailed food recall interview for the previous month.
    Results: There were statistical differences in the allelic distribution of FokI and TaqI between osteoporotic patients and controls. After adjustment for age, BMI, calcium and vitamin D intakes, statistical associations were found between TaqI VDR gene polymorphisms and BMD and were weakly correlated with serum concentrations of osteocalcin, alkaline phosphatase.
    Conclusions: Vitamin D receptor gene alleles predict the bone density and bone turnover. Our results showed that a significant difference in frequency of the VDR allelic distribution in comparison with the Asian population.

    Conference Organiser - ICMS Pty Ltd