Abstract for presentation at 11th International Congress of Human Genetics

Genome-wide microRNA and mRNA expression signatures in mouse ES and differentiated cells

  • Caifu Chen, Applied Biosystems, United States
  • Dana Ridzon, Applied Biosystems, United States
  • Dr Julie Blake, Applied Biosystems, United States
  • Dr Kenneth Livak, Applied Biosystems, United States
  • Dr Karl Guegler, Applied Biosystems, United States
  • Dr William Strauss, University of Colorado-Boulder, United States

    • TaqMan® miRNA assays were used to quantify a total of 248 microRNAs (miRNAs) in 13 mouse embryonic stem (ES) cell lines, 30 differentiated embryoid bodies (EBs) and 6 mouse tissues. MicroRNA expression profiles can classify ESs, differentiated EBs and adult tissues. We have identified ES- and differentiation-specific miRNAs that could be used as biomarkers to determine ES cell identity and to monitor the differentiation. There exists a highly conserved miRNA expression signature in 13 ES lines. Only ¼ miRNA genes are highly expressed in ES cells, and during development an increasingly elaborate miRNA signature is expressed. The stem cell specific expression of a small set of miRNAs is lost in an apparently coordinate fashion during development and does not reappear in any somatic lineage. Based on the elucidation of this regulated miRNA molecular signature, it seems likely that there is a significant role for miRNA action in the early embryo. Of 33,381mRNAs probed in an AB 1700 microarray, 452 are differentially expressed between ES cell lines and EBs. Identification of these ES differentiation-related miRNA and mRNA marker genes could be indirectly used to uncover possible miRNA’s target(s).

    Conference Organiser - ICMS Pty Ltd