Abstract for presentation at 11th International Congress of Human Genetics

Molecular Cloning and Characterisation Of Four Phosphate Transporters CDNA In Hoop Pine Trees (Araucaria Cunninghamii)

  • Mr Issa Medraj, Griffith University, Australia
  • Prof Frank Smith, University of Queensland, Australia
  • Prof Zhihong Xu, Griffith University, Australia
  • Prof Jane Hughes, Griffith University, Australia

    • Araucaria cunninghamii is an important Australian native softwood tree species, which grows in a range of different soil types. Phosphorus is one of the plant major limiting nutrients that are least available in the soil. Genes encoding phosphate transporters have been isolated from a number of plant species. In this study, four different cDNA sequences exhibiting significant sequence identity to known phosphate transporter genes were isolated from hoop pine trees. These genes were found to be different from a group of 6 genes isolated recently from hoop pine trees. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) analysis was used to study the effect of phosphate deprivation on the level of expression of these genes in different plant organs (leaves, stems and roots), while the 18S rRNA was used as an internal control gene. The level of expression of these genes was correlated with the total phosphate concentration (mineral analysis) in different plant organs grown in different phosphate concentration conditions.

    Conference Organiser - ICMS Pty Ltd