Abstract for presentation at 11th International Congress of Human Genetics

Reduced Drg-1 expression in progression of human prostate carcinoma

  • Prof DC Chu, Graduate Institute of Medical Biotechnology, Chang Gung University, Taiwan
  • Mr SF Chen, Graduate Institute of Medical Biotechnology, Chang Gung University, Taiwan
  • Dr CK Chuang, Division of Urology, Department of Surgery, Chang Gung Memorial Hospital, Lin-kou, Taiwan
  • Dr CF Sun, Department of Clinical Pathology, Chang Gung Memorial Hospital, Lin-kou, Taiwan
  • Prof HH Juang, Department of Anatomy, Chang Gung University, Taiwan

    • It was reported that Differentiation related gene-1 (Drg-1) involved in carcinogenesis, cell differentiation, androgen response, hypoxia, and N-myc pathway. In this study we aimed to investigate whether Drg-1 gene participated in prostate oncogenesis and to evaluate whether this gene expression could be a potential molecular marker for prostate cancer detection or prognosis index after treatment. Drg-1 mRNA extracted from prostate cell lines and needle biopsy samples were quantitatively analyzed with real-time quantitative PCR, while western blot was conducted to analyze DRG-1 protein expression. Immunohistochemical staining was performed to ascertain DRG-1 protein expression in these pathological samples studied. Data showed that in cell models, both mRNA levels and protein levels of Drg-1 gene were higher in normal prostate cells than in prostate cancer cells. In addition, reduced Drg-1 mRNA and protein levels were found in high grade prostate cancer tissues (Gleason score >7), comparing to the low grade tissues (Gleason score ≦ 7). It was concluded that the expression of Drg-1 gene was down-regulated in human prostate cancer as it progressed.

    Conference Organiser - ICMS Pty Ltd